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KMID : 0359319920320040597
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Korean Journal of Veterinary Research
1992 Volume.32 No. 4 p.597 ~ p.603
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Restriction endonuclease analysis of canine parvovirus DNA isolated in Korea
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박종현/Park, Jong Hyeon
송재영/안수환/전무형/이중복/현방훈/Song, Jae Young/An, Soo Hwan/Jun, Moo Hyung/Lee, Jung Bok/Hyun, Bang Hun
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Abstract
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To elucidate the molecular genetical properties of the canine parvoviruses isolated from the diseased puppies in the regions of Kyunggi and Chungnam provinces, the replicative form (RF) DNA of four field isolates were compared with those of two attenuated vaccine strains and a reference strains of CPV by restriction endonuclease analysis (REA). REA by Hinf I showed that three CPV isolates except CPV-V15 had an identical banding pattern with two vaccine strains, one standard strain and feline panleukopenia virus (FPLV). In CPV-V15 strain the fourth fragment of DNA with 800 bp was deleted. REA by Bgl II and Pst I indicated that CPV-V15 and FPLV had a bigger second fragment than those of the other strains of CPV. Meanwhile REA by Bam HI revealed that all the field isolates and vaccine strains used in this experiment showed similar banding patterns.
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KEYWORD
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